Question Eggshell DNA sexing

XBRIT
Fuzzy Dinosaur Stage
Posts: 82
Joined: Tue Feb 21, 2017 9:27 pm
Location: North of Napanee
x 119

Eggshell DNA sexing

Post by XBRIT » Tue Apr 18, 2017 9:08 pm

Does anyone have experience having their poultry DNA sexed via eggshell. Can anyone recommend a reliable company in Canada?
0

User avatar
WLLady
Stringy Old Soup Pot Hen of a Moderator
Posts: 3850
Joined: Mon Dec 07, 2015 9:55 pm
Location: Rural near West Lorne and Glencoe
x 5261

Eggshell DNA sexing

Post by WLLady » Wed Apr 19, 2017 3:22 pm

http://www.healthgene.com/
i do not have any experience with them, but they're in ontario near toronto.

for the curious out there, they sex the chicks by using the membrane that is stuck to the inside of the shell after the chick hatches.
it's a relatively simple PCR reaction...i imagine it's done using primers specific for one or two sex linked genes, probably on the w chromosome....girls would be positive for genes on both Z and W, and boys would be positive only for Z genes because they don't carry W.....
4
:giraffe: Pet quality wheaten/blue wheaten ameraucanas, RIRs, welsummers, barred rocks; Pet and Show quality wheaten marans (marans 5 eggs); Projects on the go: rhodebars, welbars, black tailed buff marans (marans 5 eggs)

User avatar
Killerbunny
Poultry Guru - chick level
Posts: 3384
Joined: Sat Dec 12, 2015 12:04 pm
Location: Brockville
x 3728

Eggshell DNA sexing

Post by Killerbunny » Wed Apr 19, 2017 5:00 pm

SO I could set a system up in the basement. Shouldn't be too hard I used to do it for E.coli and Salmonella! Just a bit pricey I guess.
4
:iheartpto:
Beltsville Small White turkeys.
Trio of Blue Columbian Wyandottes
Mutt chickens for eggs
RIP Lucky the Very Brave Splash Wyandotte rooster.
RIP little Muppet the rescue cat.
:turkey:

:bat:

User avatar
WLLady
Stringy Old Soup Pot Hen of a Moderator
Posts: 3850
Joined: Mon Dec 07, 2015 9:55 pm
Location: Rural near West Lorne and Glencoe
x 5261

Eggshell DNA sexing

Post by WLLady » Wed Apr 19, 2017 10:09 pm

Worst part of the cost is the polymerase. Primers and dNTPs are dirt cheap. The enzyme comes with patent costs so ultra price gouging. I could do this kind of thing in my sleep-i do the technique at work all the time.
1
:giraffe: Pet quality wheaten/blue wheaten ameraucanas, RIRs, welsummers, barred rocks; Pet and Show quality wheaten marans (marans 5 eggs); Projects on the go: rhodebars, welbars, black tailed buff marans (marans 5 eggs)

XBRIT
Fuzzy Dinosaur Stage
Posts: 82
Joined: Tue Feb 21, 2017 9:27 pm
Location: North of Napanee
x 119

Eggshell DNA sexing

Post by XBRIT » Wed Apr 19, 2017 10:38 pm

WLLady wrote:QR_BBPOST Worst part of the cost is the polymerase. Primers and dNTPs are dirt cheap. The enzyme comes with patent costs so ultra price gouging. I could do this kind of thing in my sleep-i do the technique at work all the time.
O.K. I have 3 eggshells bagged and more to come (I hope) So when can we get going, actually I think you will get lots of business from the folks on PTO. Well you did say the magic words "dirt cheap".:)
0

User avatar
thegawd
Head Cockerel-Moderator
Posts: 3381
Joined: Mon Dec 07, 2015 10:30 pm
Location: Port Lambton
x 3212

Eggshell DNA sexing

Post by thegawd » Thu Apr 20, 2017 10:31 am

So at $15 / test whats cheaper... feeding all the birds for 3+ months until we can sex them or having them tested so the boys can be segregated into a different pen and cared for separately or sold? Hmmmm how much does it cost to feed a chick for 3 months? LOL.

I think this dna test could be quite handy and worth it for anyone who cant keep or dont want any cockerals.

Im quite suprised that its only $15 / test!
0
Al

Home Grown Poultry

User avatar
Ontario Chick
Poultry Guru
Posts: 2645
Joined: Sat Dec 12, 2015 10:12 am
Location: Carp - West Ottawa
x 4152

Eggshell DNA sexing

Post by Ontario Chick » Thu Apr 20, 2017 10:43 am

Price has certainly gone down, I know people who had peacocks and parrots tested, used to be $25 and up
0
Keeper of Blue & Columbian Wyandottes, Blue Ameraucanas

User avatar
WLLady
Stringy Old Soup Pot Hen of a Moderator
Posts: 3850
Joined: Mon Dec 07, 2015 9:55 pm
Location: Rural near West Lorne and Glencoe
x 5261

Eggshell DNA sexing

Post by WLLady » Thu Apr 20, 2017 10:50 am

When i retire maybe i will start up a company...but i think the merit with growing out is to make sure you get the type you like...not just boy or girl...this genetics will tell you boy or girl...but not the number of points on the comb or split wing or brown or whatever....
I still am waiting to see the details of the before hatch sexing....
0
:giraffe: Pet quality wheaten/blue wheaten ameraucanas, RIRs, welsummers, barred rocks; Pet and Show quality wheaten marans (marans 5 eggs); Projects on the go: rhodebars, welbars, black tailed buff marans (marans 5 eggs)

User avatar
poultry_admin
Site Admin
Posts: 1908
Joined: Mon Dec 07, 2015 8:27 pm
Location: West Lorne, Ontario
x 1537
Contact:

Eggshell DNA sexing

Post by poultry_admin » Thu Apr 20, 2017 10:51 am

I think you guys are barking up the wrong tree. When the chick is out, you can check the chick which sex it is.
https://en.wikipedia.org/wiki/Chick_sexing
You need this test to check the eggs before they take up serious time/space in the incubator.
0

User avatar
WLLady
Stringy Old Soup Pot Hen of a Moderator
Posts: 3850
Joined: Mon Dec 07, 2015 9:55 pm
Location: Rural near West Lorne and Glencoe
x 5261

Eggshell DNA sexing

Post by WLLady » Thu Apr 20, 2017 10:54 am

So-we need permits for biological material from cfia and public health and biohazard level 2 permits and a commercial workspace...and at least 5 pcr machines. I would farm out sequencing to another company for fee, chemical purchase permits, hazmat wsste disposal permits....employees (2 plus an admin plus a safety officer), payroll and thats it. Oh and money....
4
:giraffe: Pet quality wheaten/blue wheaten ameraucanas, RIRs, welsummers, barred rocks; Pet and Show quality wheaten marans (marans 5 eggs); Projects on the go: rhodebars, welbars, black tailed buff marans (marans 5 eggs)

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest